USB-6501 does NOT support programmable power-up states

Power-on state (default state when power-on) : Input (high impedance)

It will always default all lines to be high impedance inputs, not support change the default state, even input to output.

How to resolve this issue from product? 

1. Change your application hardware design. Use for drive relay or LED, change to low level is enable.

2.Configure your DIO tasks at the beginning of application, what the means is default DIO states by creating or releasing the DIO task.

Detailed discussion as below link, http://forums.ni.com/t5/Measurement-Studio-for-VB6/Use-DAQmxSetDigitalPowerUpStates-In-VB6-for-USB-6501/m-p/584436